| Tournament details | |
|---|---|
| Country |  Scotland | 
| Teams | 163 | 
| Defending champions | Kilwinning Rangers | 
| Final positions | |
| Champions | Whitburn | 
| Runner-up | Johnstone Burgh | 
| Tournament statistics | |
| Matches played | 162 (not including replays) | 
The 1999–2000 Scottish Junior Cup was a competition in Scottish Junior football. It was won by Whitburn; they defeated Johnstone Burgh 4–3 on penalties after drawing 2–2 in the final.
First round
These ties were scheduled to take place on Saturday 9 October 1999.
| Home Team | Score | Away Team | 
|---|---|---|
Second round
These ties were scheduled to take place on Saturday 6 November 1999.
| Home Team | Score | Away Team | 
|---|---|---|
Third round
These ties were scheduled to take place on Saturday, 4 December 1999
| Home Team | Score | Away Team | 
|---|---|---|
Fourth round
These ties were scheduled to take place on Saturday, 15 January 2000.
| Home Team | Score | Away Team | 
|---|---|---|
Fifth round
These ties were scheduled to take place on Saturday, 12 February 2000.
| Home Team | Score | Away Team | 
|---|---|---|
| Benburb | 3 – 0 | Larkhall Thistle | 
| Cumnock Juniors | 3 – 0 | Kilbirnie Ladeside | 
| Johnstone Burgh | 2 – 1 | Glenafton Athletic | 
| Lanark United | 2 – 0 | Thornton Hibs | 
| Maybole Juniors | 3 – 0 | Arbroath Sporting Club | 
| Neilston | 2 – 3 | Whitburn | 
| Newtongrange Star | 2 – 0 | Sunnybank | 
| Shotts Bon Accord | 2 – 2 | Carnoustie Panmure | 
| replay | 2 – 1 | 
Quarter finals
These ties were played on Saturday 11 March 2000.
| Home Team | Score | Away Team | 
|---|---|---|
| Benburb | 2 – 1 | Newtongrange Star | 
| Johnstone Burgh | 2 – 1 | Cumnock Juniors | 
| Lanark United | 0 – 2 | Shotts Bon Accord | 
| Whitburn | 3 – 1 | Arbroath Sporting Club | 
Semi-finals
These ties were played on 28 April 2000 & 5 May 2000, respectively.
| Home Team | Score | Away Team | Venue | 
|---|---|---|---|
| Benburb | 1 – 1 | Whitburn | |
| Johnstone Burgh | 2 – 2 | Shotts Bon Accord | 
Final
The final took place on Sunday 28 May 2000.
| Home Team | Score | Away Team | Venue | 
|---|---|---|---|
| Whitburn | 2 – 2 | Johnstone Burgh | Firhill Stadium, Partick Thistle | 
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