The following highways are numbered 316:

Brazil

• BR-316

Canada

• Nova Scotia Route 316
• Prince Edward Island Route 316
• Saskatchewan Highway 316

China

• China National Highway 316

Costa Rica

• National Route 316

India

• National Highway 316 (India)

Japan

• Japan National Route 316

United States

• Connecticut Route 316
• Florida State Road 316 (former)
• Georgia State Route 316
• Illinois Route 316 (former)
• Indiana State Road 316 (former)
• Iowa Highway 316
• Kentucky Route 316
• Louisiana Highway 316
• Maryland Route 316
• Minnesota State Highway 316
• Mississippi Highway 316
• New York:
  • New York State Route 316
  • County Route 316 (Erie County, New York)
• Ohio State Route 316
• Pennsylvania Route 316
• Puerto Rico Highway 316
• Tennessee State Route 316
• Texas State Highway 316
  • Texas State Highway Spur 316
  • Farm to Market Road 316
• Utah State Route 316
• Virginia State Route 316
• Wyoming Highway 316