Fluorescent dyeColormass (g/mol)Absorb (nm)Emit (nm)ε (M−1cm−1)
FluoProbes 390violet34339047924 000
FluoProbes 488green80449351985 000
FluoProbes 532yellow765532553117 000
FluoProbes547Horange736557574150 000
FluoProbes 594red1137601627120 000
FluoProbes647Hfar-red761653674250 000
FluoProbes 682far-red853690709140 000
FluoProbes 752near-IR879748772270 000
FluoProbes 782near-IR976783800170 000
Abs = absorption maximum#, Em = emission maximum# ..................................[1]
ε = molar extinction coefficient

The FluoProbes series of fluorescent dyes were developed by Interchim to improve performances of standard fluorophores. They are designed for labeling biomolecules, cells, tissues or beads[2] in advanced fluorescent detection techniques.

  FluoProbes dyeColorLight sources (spectral line)
FluoProbes 390violetDiode laser
FluoProbes 488
Fluorescein(FITC)/Cy2
cyanArgon laser (488.0nm), Krypton laser (482.5nm)
FluoProbes 532yellowHelium–neon laser (632.8nm)
FluoProbes 547H
TRITC/Cy3
orangeArgon laser (528.7nm)
FluoProbes 594
SR101/TR
redArgon laser (528.7nm)
FluoProbes 647H
Cy5
far redKrypton laser (647.1nm), Laser (633nm)
[9]

Similar lines of fluorescent dyes provide an alternative to the FluoProbes Dyes.

References

  1. "FluoProbes Dyes" (PDF). Interchim. 2010. Retrieved 2010-03-04.
  2. Article Savina A. ; Cell 126, 205–218, July 14, 2006 (Phagosome Neutrality in Host Defense)
  3. Lightning technology from Innova BioSciences
  4. Article Brunner ; Molecular & Cellular Proteomics 2007, 6.6, pp.1007-1017
  5. AnnexinV-FluoProbes488 comparison in FCM
  6. FluoProbes labeling agent
  7. FluoProbes488 comparison to FITC, Cyanine2
  8. FluoProbes547H comparison in Confocal Microscopy
  9. "FluoProbes Dyes" (PDF). Interchim. 2010. Retrieved 2010-03-01.
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.