Guppy is a surname. Notable people with the surname include:
People
- Darius Guppy, public school fraudster
- Eileen Guppy (1903–1980), British geologist
- Henry B. Guppy (1854–1926), British botanist
- Henry Guppy (librarian) (1861–1948), British librarian
- John Guppy (1874–1937), fisherman, farmer and political figure in Newfoundland
- Lechmere Guppy (1836–1916), British naturalist; discoverer and namesake of the fish
- Sarah Guppy (1770–1852), British inventor
- Shusha Guppy (1935–2008), author, editor, singer and filmmaker
- Steve Guppy (born 1969), English retired footballer
Fictional characters
- Mr (William) Guppy, from the novel Bleak House
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