Mr. Zero may refer to:

• Mr. Freeze or Mr. Zero, a character in comic books published by DC Comics
• "Mr. Zero" (song), a song by Keith Relf
• Mr. Zero, an alias of Urbain Ledoux (1874-1941), activist for the unemployed
• Mister Zero, pseudonym of John Picard, guitarist in the Canadian band The Kings
• Mr. Zero, the protagonist of the play The Adding Machine by Elmer Rice
• Mr. 0, an alias of Sir Crocodile, an antagonist from the manga One Piece

See also

• Mr. O, a Canadian children's music television series