Pine Island may refer to:
- Pine Island, Calhoun County, Florida, an unincorporated community near Blountstown
- Pine Island, Hernando County, Florida, a census-designated place near Spring Hill
- Pine Island (Lee County, Florida), an island near Cape Coral
- Pine Island Center, Florida, a census-designated place on that island
- Pine Island National Wildlife Refuge on that island
- Pine Island Sound west of that island
- Pine Island Ridge, Florida, a former census-designated place, now part of Davie, Broward County
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