Witkin is a surname. Notable people with the surname include:

• Andrew Witkin (1952–2010), American computer scientist who made major contributions in computer vision and computer graphics
• Beatrice Witkin (1916-1990), American composer and pianist
• Bernard E. Witkin (1904–1995), founder of the California law treatise Witkin's
• Brian Witkin, founder and former president of Pacific Records
• Christian Witkin (born 1966), New York-based fashion and portrait photographer
• Evelyn M. Witkin (1921–2023), American geneticist whose research has been widely influential in DNA mutagenesis and DNA repair
• Herman Witkin (1916–1979), American psychologist who specialized in the spheres of cognitive psychology and learning psychology
• Isaac Witkin (1936–2006), internationally renowned modern sculptor, was born in Johannesburg, South Africa
• Jerome Witkin (born 1939), American figurative artist whose paintings often deal with political and cultural themes
• Joel-Peter Witkin (born 1939), American photographer who lives in Albuquerque, New Mexico
• Morton Witkin (1895–1973), American lawyer and politician