The following table lists all of the cities in the Midwestern United States with at least 100,000 people. These numbers were taken directly from the United States Census Bureau.^[1] Note that only people living in the city itself are counted. People living in suburbs are not included.

Rank	City	State	Census Population		Change
			2020	2010
1	Chicago	Illinois	2,746,388	2,695,598	+1.9%
2	Columbus	Ohio	905,748	787,033	+15.1%
3	Indianapolis	Indiana	887,642	820,445	+8.2%
4	Detroit	Michigan	639,111	713,777	−10.5%
5	Milwaukee	Wisconsin	577,222	594,833	−3.0%
6	Kansas City	Missouri	508,090	459,787	+10.5%
7	Omaha	Nebraska	486,051	408,958	+18.9%
8	Minneapolis	Minnesota	429,954	382,578	+12.4%
9	Wichita	Kansas	397,532	382,368	+4.0%
10	Cleveland	Ohio	372,624	396,815	−6.1%
11	Saint Paul	Minnesota	311,527	285,068	+9.3%
12	Cincinnati	Ohio	309,317	296,943	+4.2%
13	St. Louis	Missouri	301,578	319,294	−5.5%
14	Lincoln	Nebraska	291,082	258,379	+12.7%
15	Toledo	Ohio	270,871	287,208	−5.7%
16	Madison	Wisconsin	269,840	233,209	+15.7%
17	Fort Wayne	Indiana	263,886	253,691	+4.0%
18	Des Moines	Iowa	214,133	203,433	+5.3%
19	Grand Rapids	Michigan	198,917	188,040	+5.8%
20	Overland Park	Kansas	197,238	173,372	+13.8%
21	Sioux Falls	South Dakota	192,517	153,888	+25.1%
22	Akron	Ohio	190,469	199,110	−4.3%
23	Aurora	Illinois	180,542	197,899	−8.8%
24	Springfield	Missouri	169,176	159,498	+6.1%
25	Kansas City	Kansas	156,607	145,786	+7.4%
26	Joliet	Illinois	150,362	147,433	+2.0%
27	Naperville	Illinois	149,540	141,853	+5.4%
28	Rockford	Illinois	148,655	152,871	−2.8%
29	Olathe	Kansas	141,290	125,872	+12.2%
30	Warren	Michigan	139,387	134,056	+4.0%
31	Cedar Rapids	Iowa	137,710	126,326	+9.0%
32	Dayton	Ohio	137,644	141,527	−2.7%
33	Sterling Heights	Michigan	134,346	129,699	+3.6%
34	Topeka	Kansas	126,587	127,473	−0.7%
35	Columbia	Missouri	126,254	108,500	+16.4%
36	Fargo	North Dakota	125,990	105,549	+19.4%
37	Ann Arbor	Michigan	123,851	113,934	+8.7%
38	Independence	Missouri	123,011	116,830	+5.3%
39	Rochester	Minnesota	121,395	106,769	+13.7%
40	Evansville	Indiana	117,298	117,429	−0.1%
41	Elgin	Illinois	114,797	108,188	+6.1%
42	Springfield	Illinois	114,394	116,250	−1.6%
43	Peoria	Illinois	113,150	115,007	−1.6%
44	Lansing	Michigan	112,644	114,297	−1.4%
45	Dearborn	Michigan	109,976	98,153	+12.0%
46	Green Bay	Wisconsin	107,395	104,057	+3.2%
47	South Bend	Indiana	103,453	101,168	+2.3%
48	Davenport	Iowa	101,724	99,685	+2.0%
49	Lee's Summit	Missouri	101,108	91,364	+10.7%
50	Clinton Township	Michigan	100,513	96,796	+3.8%

References