du Toit is an Afrikaans surname of French origin, originally from François du Toit, a Huguenot who moved to South Africa in 1686. It translates as "of the roof". People with the surname include:

• Alexander du Toit (1878–1948), South African geologist
• Anri du Toit aka Yolandi Visser, South African musician
• Braam du Toit (born 1981), South African composer
• Christiaan du Toit (1901–1982), South African military commander
• Daniel du Toit (1871–1959), South African astronomer
• Dirk du Toit (1943–2009), South African politician
• Elize du Toit (born 1981), South African born, British actress
• Flooi du Toit (1869–1909), South African cricketer
• Francois Jacobus du Toit (1897–1961), South African journalist and economist
• Gaffie du Toit (born 1976), South African rugby union footballer
• Gerard'd du Toit, South African composer and conductor
• Jané du Toit (born 1975), Namibian rugby union footballer
• Lydia Lindeque (1916–1997), South African actor
• Natalie du Toit (born 1984), South African swimmer
• Nick du Toit, South African arms dealer
• Paul du Toit (1965–2014), South African artist
• Pieter-Steph du Toit (born 1992), South African rugby union player
• Rachel Alida du Toit (1916–1997), South African actor
• Sarel Petrus du Toit (1864-1930), Second Boer War Boer general
• Simoné du Toit (born 1988), South African shot putter
• Stephanus Jacobus du Toit (1847–1911), South African Afrikaans language pioneer
• Wikus du Toit, (born 1972), South African actor

Other uses

• Du Toit Mountains, Antarctica
• Du Toits Peak, Western Cape, South Africa
• Du Toit's torrent frog, Arthroleptides dutoiti, species in the family Petropedetidae
• 57P/du Toit–Neujmin–Delporte, comet
• Du Toitcrater, on Mars at coordinates 71° 48′ 0″ S, 49° 36′ 0″ W.
• Dutoitia, a genus of Devonian rhyniophyte, named after the South African geologist Alex du Toit