GIPC3
Identifiers
AliasesGIPC3, C19orf64, DFNB15, DFNB72, DFNB95, GIPC PDZ domain containing family member 3
External IDsOMIM: 608792 MGI: 2387006 HomoloGene: 77068 GeneCards: GIPC3
Orthologs
SpeciesHumanMouse
Entrez

126326

209047

Ensembl

ENSG00000179855

ENSMUSG00000034872

UniProt

Q8TF64

Q8R5M0

RefSeq (mRNA)

NM_133261

NM_148951

RefSeq (protein)

NP_573568

NP_683753

Location (UCSC)Chr 19: 3.59 – 3.59 MbChr 10: 81.34 – 81.34 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

PDZ domain-containing protein GIPC3 is a protein that in humans is encoded by the GIPC3 gene.[5][6] GIPC3 is a member of the GIPC (GAIP-interacting protein C terminus) gene family that also includes GIPC1 and GIPC2.[7] The encoded protein, GIPC3, features a centrally located PDZ domain, which is flanked on each side by a single GIPC-homology domain.[8]

Function

GIPC3 is thought to be important for acoustic signal acquisition and propagation in hair cells of the mammalian cochlea.

Gene

The human GIPC3 gene is located on the short arm of chromosome 19 at p13.3. The locus extends over about 8 kbp and contains the six coding exons that give rise to an open reading frame of 639 nucleotides encoding the GIPC3 protein of 312 amino acids. A single PDZ domain is located at amino acid position 122-189. In the mouse, Gipc3 is located on chromosome 10 at cytogenetic band qC1. The genomic region covers a distance of 5.5 kbp. The six coding exons encode a protein of 297 amino acids. The PDZ domain is located at amino acid position 107-174.

Genetics

In the mouse, a missense mutation in Gipc3 (c.343G>A) leads to a non-synonymous amino acid replacement (p.G115R) in the loop connecting two beta strands of the PDZ domain. Glycine 115 is conserved in all GIPC proteins.[9] Missense (c.785C>T; p. L262R) and nonsense (c.903G>A, p.W301X) mutations in human GIPC3 cause congenital sensorineural hearing impairment in families segregating non-syndromic hearing loss DFNB15 and DFNB95.

Phenotypes

Mice of the Black Swiss strain develop early-onset slowly progressing sensorineural hearing loss. A genetic study identified two quantitative trait loci (QTL) that control hearing function. One QTL, named age-related hearing loss 5 (ahl5) localizes to chromosome 10 and accounted for ca. 60% of the variation in hearing thresholds. A second QTL, ahl6, localized to chromosome 18 and has a smaller effect size.[10] Besides their hearing impairment, Black Swiss mice also are hypersensitive to acoustic stimulation, reacting with seizures (audiogenic seizures) to loud white noise.[11] A genetic locus conferring susceptibility was identified (juvenile audiogenic monogenic seizures1, jams1) on chromosome 10. A positional cloning approach aimed to decipher the genetic basis of both the hearing loss and audiogenic seizure susceptibility subsequently identified the glycine to arginine substitution in Gipc3 as the underlying cause.

In humans, individuals with the p.W301X missense mutation (DFNB95) exhibit bilateral sensorineural hearing loss with threshold shifts of 70-80 dB hearing levels as early as 11 months of age.

Interactions

The PDZ domain of GIPC family proteins interact with:[7]

  • Frizzled-3 (FZD3) class of WNT receptor,
  • insulin-like growth factor-I receptor (IGF1R),
  • receptor tyrosine kinase TrkA,
  • TGF-beta type III receptor (TGF-beta RIII),
  • integrin alpha6A (ITGA6),
  • transmembrane glycoprotein TPBG, and
  • RGS19/RGS-GAIP.

See also

  • GIPC PDZ domain containing family, member 1, GIPC1
  • GIPC PDZ domain containing family, member 2, GIPC2

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000179855 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000034872 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ↑ "Entrez Gene: GIPC PDZ domain containing family".
  6. ↑ Saitoh T, Mine T, Katoh M (March 2002). "Molecular cloning and characterization of human GIPC3, a novel gene homologous to human GIPC1 and GIPC2". Int. J. Oncol. 20 (3): 577–82. doi:10.3892/ijo.20.3.577. PMID 11836571.
  7. 1 2 Katoh M (June 2002). "GIPC gene family (Review)". Int. J. Mol. Med. 9 (6): 585–9. doi:10.3892/ijmm.9.6.585. PMID 12011974.
  8. ↑ Katoh, M. (2002). "GIPC gene family (Review)". International Journal of Molecular Medicine. 9 (6): 585–589. doi:10.3892/ijmm.9.6.585. PMID 12011974.
  9. ↑ Charizopoulou N, Lelli A, Schraders M, Ray K, Hildebrand MS, Ramesh A, Srisailapathy CR, Oostrik J, Admiraal RJ, Neely HR, Latoche JR, Smith RJ, Northup JK, Kremer H, Holt JR, Noben-Trauth K (February 2011). "Gipc3 mutations associated with audiogenic seizures and sensorineural hearing loss in mouse and human". Nat Commun. 2 (2): 201. Bibcode:2011NatCo...2..201C. doi:10.1038/ncomms1200. PMC 3105340. PMID 21326233.
  10. ↑ Drayton, M.; Noben-Trauth, K. (2006). "Mapping quantitative trait loci for hearing loss in Black Swiss mice". Hearing Research. 212 (1–2): 128–139. doi:10.1016/j.heares.2005.11.006. PMID 16426780. S2CID 23928550.
  11. ↑ Misawa, H.; Sherr, E. H.; Lee, D. J.; Chetkovich, D. M.; Tan, A.; Schreiner, C. E.; Bredt, D. S. (2002). "Identification of a monogenic locus (jams1) causing juvenile audiogenic seizures in mice". Journal of Neuroscience. 22 (23): 10088–10093. doi:10.1523/JNEUROSCI.22-23-10088.2002. PMC 6758732. PMID 12451109.

Further reading

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