GLYAT
Identifiers
AliasesGLYAT, ACGNAT, CAT, GAT, glycine-N-acyltransferase
External IDsOMIM: 607424 MGI: 2147502 HomoloGene: 64840 GeneCards: GLYAT
Orthologs
SpeciesHumanMouse
Entrez

10249

107146

Ensembl

ENSG00000149124

ENSMUSG00000063683

UniProt

Q6IB77

Q91XE0

RefSeq (mRNA)

NM_201648
NM_005838

NM_145935

RefSeq (protein)

NP_005829
NP_964011

NP_666047

Location (UCSC)Chr 11: 58.64 – 58.73 MbChr 19: 12.61 – 12.63 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Glycine-N-acyltransferase, also known as GLYAT, is an enzyme which in humans is encoded by the GLYAT gene.[5][6]

Function

The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria primarily in liver and kidney. The glycine N-acyltransferase enzyme is involved in the detoxification of a wide range of xenobiotic and endogenous metabolites. These include benzoic acid, a compound found in fruits and vegetables and used in medicine and foodstuffs as a preservative; salicylic acid, a metabolite of aspirin; and several endogenous metabolites. The diversity is demonstrated by the wide range of acylglycines excreted in the urines of patients with defects of organic acid metabolism. No defect of glycine N-acyltransferase has yet been described, but it has been demonstrated that there is significant inter individual variation in glycine conjugation capacity. Human glycine N-acyltransferase isoform a is a 296 amino acid protein translated from mRNA transcript splice variant 1. It is encoded by exons 2 to 6 of the mRNA transcript.[5]

Molecular weight

The literature reports it to be approximately 30 kDa,[6] or approximately 27 kDa.[7] The predicted size is 33.9 KDa. For the bovine enzyme a range of sizes between approximately 33 kDa and about 36 KDa is reported (Nandi, 1979, Vessey, 1992, van der Westhuizen, 2000). The predicted size of bovine GLYAT based on its sequence (accession number nm: 177486), is 33.9 kDa. This compares well to the experimentally determined sizes[7][8][9]

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000149124 - Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000063683 - Ensembl, May 2017
  3. ↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. 1 2 "Entrez Gene: GLYAT glycine-N-acyltransferase".
  6. 1 2 Mawal YR, Qureshi IA (December 1994). "Purification to homogeneity of mitochondrial acyl coa:glycine n-acyltransferase from human liver". Biochem. Biophys. Res. Commun. 205 (2): 1373–9. doi:10.1006/bbrc.1994.2817. PMID 7802672.
  7. 1 2 van der Westhuizen FH, Pretorius PJ, Erasmus E (2000). "The utilization of alanine, glutamic acid, and serine as amino acid substrates for glycine N-acyltransferase". J. Biochem. Mol. Toxicol. 14 (2): 102–9. doi:10.1002/(SICI)1099-0461(2000)14:2<102::AID-JBT6>3.0.CO;2-H. PMID 10630424. S2CID 44672034.
  8. ↑ Nandi DL, Lucas SV, Webster LT (August 1979). "Benzoyl-coenzyme A:glycine N-acyltransferase and phenylacetyl-coenzyme A:glycine N-acyltransferase from bovine liver mitochondria. Purification and characterization". J. Biol. Chem. 254 (15): 7230–7. doi:10.1016/S0021-9258(18)50309-4. PMID 457678.
  9. ↑ Kelley M, Vessey DA (November 1992). "Structural comparison between the mitochondrial aralkyl-CoA and arylacetyl-CoA N-acyltransferases". Biochem. J. 288 (1): 315–7. doi:10.1042/bj2880315. PMC 1132116. PMID 1445276.

Further reading


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