Le Sueur or Lesueur is a French surname. Notable people with the surname include:

• Hubert Le Sueur (c. 1580 – 1658), French sculptor
• Jean Le Sueur (c. 1598 – 1668, also known as Abbé Saint-Sauveur), French-born Quebecois priest
• Eustache Le Sueur (1617 – 1655), painter and a founder of the French Academy
• Pierre-Charles Le Sueur (c. 1657 – 1704), French trapper and explorer of North America
• Jacques-François le Sueur (fl. 1704 – 1754), French Jesuit missionary and linguist of the Abnaki in Canada
• Jacques-Philippe Le Sueur (1759–1830), French sculptor
• Jean-François Le Sueur (1760 – 1837), French composer
• Charles Alexandre Lesueur (1778–1846), French naturalist-illustrator and explorer of Australia, Southeast Asia, and North America
• Pierre-Étienne Lesueur (fl. 1791-1810), French painter
• Marie Lesieur (1799–1890), known as Lesueur, French ballet dancer
• Georges Lesueur (1834–1910), French engineer, public works contractor, Senator of Algeria from 1888 to 1897
• Daniel Lesueur, pen name of Jeanne Lapauze, née Loiseau (1860–1920), French poet and novelist
• Arthur LeSueur (1867?–1950?), American socialist newspaper editor and Socialist mayor of Minot, North Dakota
• Florence LeSueur (1898–1991), African-American civil rights activist and the first female president of a NAACP chapter
• Meridel Le Sueur nee Wharton (1900–1996), American writer and political activist, stepdaughter of Arthur Le Sueur
• Hal LeSueur (c.1901 or 1903 – 1963), American actor and elder brother of Joan Crawford
• Lucille Fay LeSueur, real name of Joan Crawford (c.1904, 1905, 1906 or 1908 – 1977), film actress and sister of Hal
• Raoul Lesueur (1912–1981), French cyclist
• Terry Le Sueur (born 1942?), politician and Chief Minister of Jersey
• Clinton LeSueur (born 1969), Mississippi politician
• Emily LeSueur (born 1972), US synchronized swimmer
• Éloyse Lesueur (born 1988), French long jumper