The partial specific volume express the variation of the extensive volume of a mixture in respect to composition of the masses. It is the partial derivative of volume with respect to the mass of the component of interest.

where is the partial specific volume of a component defined as:

The PSV is usually measured in milliLiters (mL) per gram (g), proteins > 30 kDa can be assumed to have a partial specific volume of 0.708 mL/g.[1] Experimental determination is possible by measuring the natural frequency of a U-shaped tube filled successively with air, buffer and protein solution.[2]

Properties

The weighted sum of partial specific volumes of a mixture or solution is an inverse of density of the mixture namely the specific volume of the mixture.

See also

References

  1. Buxbaum, Engelbert (2011). "25: Centrifugation". Biophysical Chemistry of Proteins. New York, Dordrecht, Heidelberg, London: Springer. pp. 237–249. doi:10.1007/978-1-4419-7251-4_25. ISBN 978-1-4419-7250-7.
  2. Kratky, O.; Leopold, H.; Stabinger, H. (1973). "5: The determination of the partial specific volume of proteins by the mechanical oscillator technique". In Hirs, C.H.W.; Timasheff, S.N. (eds.). Meth. Enzymol. Vol. 27: Enzyme Structure Part D. pp. 98–110. ISBN 978-0-12-181890-6.


This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.