Disc one

1. "Hesitation Blues" (Traditional) (from Hot Tuna) – 5:05
2. "Know You Rider" (Traditional) (from Hot Tuna) – 4:07
3. "Winin' Boy Blues" (Jelly Roll Morton) (from Hot Tuna) – 5:31
4. "Mann's Fate" (Jorma Kaukonen) (from Hot Tuna) – 5:20
5. "Keep Your Lamps Trimmed and Burning" (Rev. Gary Davis) (from First Pull Up, Then Pull Down) – 8:15
6. "Candy Man" (Davis) (from First Pull Up, Then Pull Down) – 5:48
7. "Been So Long" (Kaukonen) (Studio Version) (from "Been So Long" single) – 3:45
8. "Keep On Truckin'" (B. Carleton) (from Burgers) – 3:40
9. "99 Year Blues" (Julius Daniels) (from Burgers) – 3:57
10. "Ode for Billy Dean" (Kaukonen) (from Burgers) – 4:50
11. "Sea Child" (Kaukonen) (from Burgers) – 5:00
12. "Water Song" (Kaukonen) (from Burgers) – 5:15
13. "I See the Light" (Kaukonen) (from The Phosphorescent Rat) – 4:15
14. "Living Just for You" (Kaukonen) (from The Phosphorescent Rat) – 3:18
15. "Easy Now" (Kaukonen) (from The Phosphorescent Rat) – 5:10
16. "Sally Where'd You Get Your Liquor From" (Davis) (from The Phosphorescent Rat) – 2:56

Disc two

1. "Hit Single #1" (Kaukonen) (from America's Choice) – 5:13
2. "Serpent of Dreams" (Kaukonen) (from America's Choice) – 6:51
3. "Sleep Song" (Kaukonen) (from America's Choice) – 4:23
4. "Funky #7" (Jack Casady, Kaukonen) (from America's Choice) – 5:47
5. "Hot Jelly Roll Blues" (Bo Carter) (from Yellow Fever) – 4:21
6. "Sunrise Dance with the Devil" (Kaukonen) (from Yellow Fever) – 4:28
7. "Bar Room Crystal Ball" (Kaukonen) (from Yellow Fever) – 6:52
8. "I Wish You Would" (Billy Boy Arnold) (from Hoppkorv) – 3:10
9. "Watch the North Wind Rise" (Kaukonen) (from Hoppkorv) – 4:39
10. "It's So Easy" (Buddy Holly, Norman Petty) (from Hoppkorv) – 2:35
11. "Song from the Stainless Cymbal" (Kaukonen) (from Hoppkorv) – 4:02
12. "Genesis" (Kaukonen) (from Double Dose) – 4:27
13. "Rock Me Baby" (Joe Josea, B. B. King) (previously unreleased) – 7:36
14. "Extrication Love Song" (Kaukonen) (from Double Dose) – 5:20

Production

• Paul Williams – producer, compilation, tape research
• Bill Thompson – compilation, assistant
• Ray Loughren – assistant, sequencer
• Jim Cooperman – assistant
• Brian Mayer – assistant
• Bill Lacey – audio restoration
• Mike Harty – digital transfer
• Michael Drexler – digital transfer
• Dalita Keumurian – project director
• Dave Cohen – essay
• Tori Larkey – vault research
• Jack Rovner – creative director
• Henry Marquez – art direction
• Grace Slick – illustrations
• Mike Diehl – design